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Fish, common carp (Cyprinus carpio) growth hormone - Cat no. GHP-7 GENE ID 109086196

Description:
Recombinant common carp (Cyprinus carpio) growth hormone (caGH) produced in E.Coli (21.4 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of 21404 Da. Recombinant common carp growth hormone is purified by chromatographic techniques, according to Fine et al. General and Comparative Endocrinology, 89,51-61 (1993).
Source:
E. coli
Physical Appearance:
White lyophilized (freeze-dried) powder.
Formulation:
The recombinant common carp growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.03% NaHCO3 adjusted to pH 8.
Solubility:
It is recommended to reconstitute the lyophilized recombinant common carp growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml. Recombinant common carp growth hormone can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability:
Lyophilized recombinant common carp growth hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution of recombinant common carp growth hormone at > 0.1 mg/ml and filter sterilization can be stored at 4C for at least two weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles.
Purity:
The purity od recombinant common carp growth hormone is greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC.
Amino Acid Sequence:
The sequence of the first seven N-terminal amino acids of recombinant common carp growth hormone was determined and was found to be Ser-Asp-Asn-Gln-Arg-Leu-Phe.
Dimers and Aggregates:
The purified recombinant common carp growth hormone (21.4K) consists of > 98% monomers as determined by gel-filtration chromatography.
Biological Activity:
Recombinant common carp growth hormone is biologically active in rat 3T3 F442A preadipocytes, though its activity is 15-fold lower compared to bovine GH, but it is equally potent in vivo in promoting carp growth (Fine et al.1993). Recombinant common carp growth hormone is capable to form a 1:2 complex with oGHR-ECD and induce proliferation of PFD-P1 cells stably transfected with rabbit growth hormone receptor, though its activity is 5-fold lower compared to bovine GH (A. Gertler, unpublished data).
Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of recombinant common carp growth hormone.
Protein content:
Protein quantitation of recombinant common carp growth hormone was carried out by two independent methods:
UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by DNAman computer analysis program of protein sequences.
Usage:
The recombinant common carp growth hormone is offered by PLR for research, laboratory or further manufacturing purposes.