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Human Glial-derived Neurotrophic Factor (hGDNF) - Cat no. CYT-12 GENE ID 2668

Description:
GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Glial Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.
GDNF is purified by proprietary chromatographic techniques.
Source:
E.Coli
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
GDNF was lyophilized after dialysis against 10 mM sodium citrate and 150mM NaCl.
Solubility:
It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions containing carrier proteins such as BSA or HSA.
Stability:
Lyophilized Glial Derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution GDNF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
Dimers and Aggregates:
Biological Activity:
The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml.
Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of bPL
Protein content:
Usage:
PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.